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Thomson S , Nelson A , White C , Rieder M 1, 3, 5, Koren G , van Uum S 1 2 Dept. of Physiology and Pharmacology, Dept. of 3 4 Anthropology, Div. of Clinical Pharmacology, Div. of Endocrinology and Metabolism, Dept. of Medicine, 5 Dept. of Paediatrics, Schulich School of Medicine and 6 Dentistry, Lawson Health Research Institute, 7 University of Western Ontario, Robarts Research 8 Institute, CIHR-GSK Chair in Paediatric Clinical 9 Pharmacology, Division of Clinical Pharmacology, University of Toronto, Toronto, Canada Corresponding Author: sthoms24 uwo Funding Source: Physicians Services Incorporated, Canadian Institutes of Health Research Background: Cortisol is a vital corticosteroid playing a key role in many systems, and is used to treat various pathological conditions. A technique developed by us allows the measurement of cortisol in hair, yielding long term assessment of cortisol concentrations in individuals compared to the short time window of traditional specimens like blood, saliva and urine. We applied this technique to mummies from the Americas, and hypothesized that the mummies will have a cortisol concentration that is stable for many years and, due to the stresses associated with life during this period, that the levels will be higher than currently seen in normal individuals. Methods: Hair was obtained from several mummies. Samples were incubated overnight in methanol to extract steroid. Methanol was evaporated and residue reconstituted in PBS. Samples were analyzed using a commercially available salivary ELISA Alpco Diagnostics ; . Results: All mummies analyzed showed detectable cortisol concentrations which were higher 234131 ng g ; than found in normal present day individuals 4933 ng g, P 0.001 ; . Concentrations in one individual are suggestive of an endocrine disorder. Conclusions: These results suggest that hair analysis provides a new method of measuring pathophysiological markers in mummies, in whom hair is well preserved. Hair analysis is now widely used in anthropology, and this technique broadens the range of tools available to piece together our history. This technique could also be used to for monitoring long term concentrations of endogenous and exogenous steroids in relation to diagnosis and treatment of various hormonally-related conditions. Keywords: Cortisol, hair, ELISA.

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71 ; NOVARTIS AG [CH CH]; Lichstrasse 35, CH-4056 Basel CH ; . only for seulement pour AT US ; 71 ; NOVARTIS PHARMA GM BH [AT AT]; Brunner Strasse 59, A-1230 Vienna AT ; . only for seulement pour AT ; 72, 75 ; BEBERNITZ , Gregory, Raym ond [US US]; 24 Lanes End, Stow, MA 01775 US ; . 74 ; GRUBB, Philip; Novartis AG, Corporate Intellectual Property, CH-4002 Basel CH ; . 81 ; ZW. 84 ; EA AZ Published Publie : c ; 51 ; C07D 285 10, A61K 31 433 11 ; W O 2004 050646 21 ; PCT GB2003 005120 22 ; 26 Nov nov 2003 26.11.2003 ; 25 ; en 30 ; 0227813.3 26 ; en 29 Nov nov 2002 29.11.2002 ; GB 13 ; A1. S. Ernst 1 , K.R.J. Chun 2 , B. Schmidt 2 , F. Ouyang 2 , P. Falk 2 , D. Baensch 2 , M. Antz 2 , K.H. Kuck 2 . 1 St. Georg Hospital, II. Med. Abteilung Cardiology Dept., Hamburg, Germany; 2 St. Georg General Hospital, II. Medical Department, Hamburg, Germany The magnetic navigation system MNS ; Niobe Stereotaxis Inc, USA ; allows remote-controlled navigation by changing the orientation of a small magnet embedded in the tip of an ablation catheter by two outer permanent magnets 0.08 Tesla ; . In addition, using a motor unit Cardiodrive, Stereotaxis ; advancing and retracting of the magnetic catheter can be performed from the control room without radiation exposure for the investigator. Using a custom-made device Siemens, Forchheim ; , we prospectively studied the amount of fluoroscopy deployed from the examination versus the control room to assess the radiation exposure for both patient and investigator. Methods: A total of 43 pts 24 f, mean age 48 15 yrs ; underwent an invasive electrophysiologic study using the MNS. After positioning of conventional diagnostic catheters, the magnetic ablation catheter Helios II, Stereotaxis ; was used to perform radiofrequency RFC ; ablation in a remote-controlled fashion. Results: Using conventional electrophysiologic criteria, the following underlying tachycardia substrates were identified: accessory pathway conduction AP ; in 10 pts, AV node reentrant tachycardia AVNRT ; in 29 and atrial flutter Afl ; in 1 pt, while 3 remained non-inducible no AP, no dual AVN ; . Besides 2 pts Afl and AP ; , pts got successfully ablated using the MNS. Median procedure duration amounted to 120 55 270 ; min using a median of 4.1 1.5 17.3 ; min of total fluoroscopy. Radiation dosage for the patient was in median 193 17 726 ; Gym2 . A median of 1 0.3 9.4 ; min of radiation was applied by the investigator positioned at the table. The remaining median of 1.6 0.1 7.3 ; min was deployed from the control room. Conclusions: Remote catheter ablation of supraventricular tachycardia using the new magnetic navigation system Niobe lowers radiation exposure for both patients and investigators. The investigator receives only about half of the overall fluoroscopy, since most of the procedure is performed remote-controlled. However, further improvement of the system catheter irrigation, 3D imaging, etc. ; is needed to address more complex arrhythmias in the future and cephalexin and biaxin, for instance, biaaxin for sinus.

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